Enzyme linked immunosorbent assay for determination of amlodipine in plasma

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Enzyme linked immunosorbent assay for determination of amlodipine in plasma.

Amlodipine is a calcium channel antagonist of the dihydropyridine group. It is effective for treating hypertension, chronic stable angina, and vasospastic angina. However, it is difficult clinically to pinpoint the maximum dosage for antihypertensive activity of the drug without having parallel data on the plasma drug concentrations. The methods for assaying amlodipine are either gas chromatogr...

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An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...

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Application of a modified human enzyme-linked immunosorbent assay kit for diagnosis of hydatidosis in sheep

Cystic hydatid disease (hydatidosis) is one of the most important zoonosis that is caused by the larval stage of Echinococcus granulosus . As its diagnosis by clinical symptoms alone is difficult and confusing, serologic diagnostic techniques are used to confirm the disease. These techniques can also be used for   epidemiologic studies. The present study was performed with a commercial human en...

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New enzyme-linked immunosorbent assay for glycocalicin in plasma.

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Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not satisfactory. Therefore, WHO recommended spe-cific serological methods using specific antigens,...

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ژورنال

عنوان ژورنال: Journal of Clinical Laboratory Analysis

سال: 2001

ISSN: 0887-8013,1098-2825

DOI: 10.1002/1098-2825(2001)15:1<47::aid-jcla10>3.0.co;2-7